• Frozen / Standard (CL145-STAN) $ 3,000

This is a cell line derived from the murine acute myeloid leukemia C1498 cell line (ATCC® TIB-49^TM). Parental C1498 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide, and 2) LV-eGFP-PGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high Fluc- and eGFP-expressing population was generated by selection using G418 and puromycin followed by selection using a methylcellulose based semi-solid medium.