Species: Mouse

Cell type: Colorectal Cancer

Transgenes: Near infrared fluorescent protein (iRFP; ex/em = 690/713 nm) with neomycin resistance (Neo) for selection with G418.

Media: DMEM, 10% FBS, 1% Pen/Strep, 0.4 mg/mL G418

Description:

CT26.WT-iRFP-Neo is a polyclonal population of the colorectal carcinoma cell line CT26.WT (ATCC® CRL-2638™) transduced with LV-iRFP-P2A-Neo (LV033) encoding the near-infrared fluorescent protein (iRFP) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide.

The lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Mycoplasma Testing:

The CT26.WT-iRFP-Neo cell line has been tested for mycoplasma contamination and is certified mycoplasma free.

Cell Line Authentication:

The parental CT26.WT cell line was authenticated and certified free of interspecies cross-contamination by short tandem repeat (STR) profiling with 27 STR loci.

Recommended uses:

In vitro: This is a high iRFP expressing cell line suitable for use as a positive control cell line in fluorescence assays to verify iRFP expression in your lentiviral transduced cells.

In vivo: CT26.WT cells form tumors post implantation into mice. The in vivo growth of these tumors may be monitored using noninvasive optical imaging.

Note: Please ensure that your optical imaging systems have the appropriate filters for detection of iRFP (ex/em = 690/713 nm).

Morphology: Low- and high-density cell morphology (200x)

Flow Cytometry for iRFP: CT26.WT-iRFP-Neo cells (red) or control (CT26.WT-Fluc-Neo; grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry (20,000 events).