Nalm6-Fluc-Neo/eGFP-Puro
- Frozen / Standard (CL150-STAN) $ 3,000
This is a cell line derived from the human B cell precursor leukemia Nalm6 cell line (ATCC® CRL-3273TM). Parental Nalm6 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus- forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide and 2) LV- eGFP-PGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high Fluc and eGFP expressing population was generated by selection using G418 and puromycin followed by selection using a methylcellulose based semi-solid medium.