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pLV-SFFV-human NIS

  • 20 ug (DNA1001) $ 1,150
  • Name of plasmid: pLV-SFFV-human NIS
  • DescriptionpHR'-SIN-SFFV-hNIS plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
  • Transgene: Human sodium iodide symporter (hNIS)
  • Proposed Use: For generation of lentiviral vector stocks encoding human NIS in a triple transfection protocol.

Plasmid Map:

lentivector, plasmid map, hNIS, NIS

Transgene Validation: Uptake assay of LV-hNIS transduced cells in the presence or absence of KClO4, an inhibitor of NIS-mediated 125I  uptake.

Uptake, hNIS, NIS

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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