pLV-SFFV-humanNIS-Neo

• Name of plasmid: pLV-SFFV-human NIS-Neo
• Description: pHR' SIN-SFFV-hNIS-IRES-Neo plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
• Transgenes: Human sodium iodide symporter (hNIS) and neomycin resistance (Neo)
• Linker: IRES
• Proposed Use: For generation of lentiviral vector stocks encoding human NIS and neomycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were trasduced with LV-hNIS-Neo and selected with G418. NIS mediated ¹²⁵I uptake was assayed in the presence or absence of KClO₄, a competitive inhibitor of iodine uptake.