pLV-SFFV-ratNIS
- 20 ug / Standard ((DNA1011-STAN)) $ 1,500
- Name of plasmid: pLV-SFFV-rat NIS
- Description: pHR'-SIN-SFFV-rNIS plasmid DNA
- Amount of DNA: 20 ug
- Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
- Transgene: Rat NIS
- Proposed Use: For generation of lentiviral vector stocks encoding rat NIS in a triple transfection protocol.
Plasmid Map:
Transgene Validation: Uptake assay of LV-rNIS transduced cells in the presence or absence of KClO4, an inhibitor of NIS-mediated 125I uptake.