pLV-SFFV-Fluc
- 20 ug / Standard ((DNA1014-STAN)) $ 1,250
- Name of plasmid: pLV-SFFV-Fluc
- Description: pHR'-SIN-SFFV-firefly luciferase plasmid DNA
- Amount of DNA: 20 ug
- Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
- Transgene: Firefly luciferase
- Proposed Use: For generation of lentiviral vector stocks encoding firefly luciferase in a triple transfection protocol.
Plasmid Map:
Transgene Validation: Luciferase assay of LV-Fluc transduced cells, imaged with a Xenogen IVIS Spectrum.