pLV-SFFV-ratNIS-Puro

• Name of plasmid: pLV-SFFV-rat NIS-Puro
• Description: pHR' SIN-SFFV-rNIS-IRES-Puro plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
• Transgenes: Rat sodium iodide sumporter (rNIS) and puromycin resistance (Puro)
• Linker: IRES
• Proposed Use: For generation of lentiviral vector stocks encoding rat NIS and puromycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were transduced with LV-rNIS-Puro and selected with puromycin. NIS mediated ¹²⁵I uptake was assayed in the presence or absence of KClO₄, a competitive inhibitor of iodine uptake.