• 20 ug / Standard ((DNA1022-STAN)) $ 1,250

• Name of plasmid: pLV-SFFV-eGFP-PGK-Puro
• Description: pHR' SIN-SFFV-eGFP-PGK-Puro plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
• Transgenes: Enhanced green fluorescent protein (eGFP) and puromycin (Puro)
• Proposed Use: For generation of lentiviral vector stocks encoding enhanced GFP and puromycin in a triple transfection protocol.

Plasmid Map:

Transgene Validation: LV-eGFP-PGK-Puro transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.