pLV-SFFV-eGFP-P2A-humanNIS

• Name of plasmid: pLV-SFFV-eGFP-P2A-human NIS
• Description: pHR' SIN-SFFV-eGFP-P2A-hNIS plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells,
• Transgenes: Enhanced green fluorescent protein (eGFP) and human sodium iodide symporter (hNIS)
• Linker: Self-cleavage peptide P2A
• Proposed Use: For generation of lentiviral vector stocks encoding enhanced GFP and human NIS in a triple transfection protocol.

Plasmid Map:

Transgene Validation:

Flow cytometry: LV-eGFP-hNIS transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.

Uptake Assay: NIS mediated ¹²⁵I uptake of LV-eGFP-hNIS transduced cells was assayed in the presence or absence of KClO₄, a competitive inhibitor of iodine uptake.