pLV-SFFV-DRD2-PGK-Puro

• Name of plasmid: pLV-SFFV-DRD2-PGK-Puro
• Description: pHR' SIN-SFFV-DRD2-PGK-Puro plasmid DNA
• Amount of DNA: 20 ug
• Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
• Transgenes: Human dopamine receptor D2 (DRD2) and puromycin resistance (Puro)
• Proposed Use: For generation of lentiviral vector stocks encoding human dopamine receptor D2 and puromycin in a triple transfection protocol.

Plasmid Map:

Transgene Validation: LV-DRD2-PGK-Puro transduced cells (red) and control cells (grey) were labeled with a spiperone derivative (DRD2 antagonist) coupled to a red fluorescent probe. After binding, cells were fixed with paraformaldehyde and analyzed by flow cytometry.