Imanis Life Sciences

Menu

pLV-SFFV-HSVTK

  • 20 ug (DNA1052) $ 1,250
  • Name of plasmid: pLV-SFFV-HSVTK
  • Description: pHR' SIN-SFFV-HSVTK plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
  • Transgene: Herpes simplex virus thymidine kinase (HSVTK)
  • Proposed Use: For generation of lentiviral vector stocks encoding herpes simplex virus thymidine kinase in a triple transfection protocol.

Plasmid Map:

plasmid map hsvtk

 

Transgene Validation: HeLaH1 cells were transduced with LV-HSVTK and assayed for uptake of 3H-penciclovir as a measure of HSVTK activity. Parental HeLaH1 cells were used as a negative control.

uptake HSVTK

    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

    What people say about Imanis

    Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

    – Dr. Raymond Hickey, Mayo Clinic

    Learning Resources