- 20 ug (DNA1068) $ 720
- Name of plasmid: pLV-SFFV-dsRed-PGK-Puro
- Description: pHR' SIN-SFFV-dsRed-PGK-Puro plasmid DNA
- Amount of DNA: 20 ug
- Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
- Transgenes: Red fluorescent protein (dsRed) and puromycin resistance (Puro)
- Proposed Use: For generation of lentiviral vector stocks encoding red fluorescent protein and puromycin resistance in a triple transfection protocol.
Transgene Validation: LV-dsRed-PGK-Puro transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.