pLV-TBG-mouseNIS

• Name of plasmid: pLV-TBG-mouse NIS
• Description: pHR'-SIN-TBG-mNIS plasmid DNA
• Amount of DNA: 20 ug
• Promoter: Liver-specific human thyroxine-binding globulin (TBG) promoter
• Transgene: Mouse sodium iodide symporter (mNIS)
• Proposed Use: For generation of liver-specific lentiviral vector stocks encoding mouse NIS in a triple transfection protocol.

Plasmid Map:

Transgene Validation: Hepa1-6 hepatoma cells were transduced with LV-TGB-mNIS at various MOI. NIS mediated ¹²⁵I uptake was assayed in the presence or absence of KClO₄, a competitive inhibitor of iodine uptake.