• 0.25 mL / Standard / Y ((OV4009-STAN)) $ 1,500

VV_(Li)-iCBG-RFP-GusA is a recombinant vaccinia virus (VV) encoding the following additional transcription units: 1) the tet-repressor (tetR)  cDNA under transcriptional control of the VV synthetic early late (pSEL) promoter, 2) the tet-operator sequence tetO followed by the click beetle green luciferase (CBG)-red fluorescence protein (RFP) fusion cDNA under transcriptional control of the VV synthetic late (pSL) promoter, and 3) the β-glucuronidase (GusA) cDNA under transcriptional control of the p11 promoter. These additional transcription units are inserted into, and disrupt the function of, the viral F14.5L, J2R (thymidine kinase), and A56R (hemagglutinin) genes, respectively. Expression of the CBG-RFP fusion protein is under the control of an inducible promoter-system, whereby addition of doxycycline induces the expression of CBG-RFP fusion protein (see diagram below). The purchased virus is ready for use in cell-killing assays or can be amplified to create additional virus stocks.