A375 (ATCC® CCL-121™) is a human melanoma cell line initiated through explant culture of a solid tumor from a 54-year-old female. The cells are adherent with an epithelial morphology. The cells form tumors following implantation into immunocompromised mice.

Route of Implantation	Mice	Tumor/Metastases	References
Intradermal	NOD/SCID	Dermal tumors, micrometastases in lung and brain	Rozenberg et al. (2010) Melanoma Res 20:361-371.
Subcutaneous	Nude	Subcutaneous tumor	White et al. (2009) Purinergic Signal 5: 327-333.
Tail vein	NSG	Lung metastases	Weber et al. (2016) Cancer Res 15: 3562-3571.
Intracardiac	Nude	Lung, liver, bone, and lymph node metastases	Huang et al. (2014) Endocrinology 155:3739-3749.

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Our A375 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our A375 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter A375 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998). 

For more information about a specific reporter and its uses, click on the photos below.