Description: Polyclonal antibody to rat NIS 

Antigen: Synthetic peptide corresponding to residues 603-618 of the rat sodium iodide symporter

Detects both the native and denatured forms of rNIS. This antibody recognizes an intracellular C-terminal epitope that is conserved between rat and mouse NIS

Host Species: Rabbit

Isotype: Not applicable. Polyclonal affinity purified antibody.

Concentration: 1 mg/mL

Species reactivity: Rat, mouse 

Tested Applications:

• Flow cytometry (1:500 dilution)
• Immunofluorescence (1:500 dilution) 
• Immunohistochemistry (1:500 dilution)
• Western Blotting (1:500 to 1:1,000 dilution) 

Selected Product References:

1. Levy et al. Proc. Natl. Acad. Sci. USA. 1997. 94:5568-5573

2. De la Vieja et al. J. Cell Sci. 2004. 117:677-687

3. Nicola et al. Am. J. Physiol. Cell Physiol. 2009. 296:C654-C662

4. Tazebay et al. Nat. Med. 2000. 6:871-878

5. Paroder-Belenitsky et al. Proc. Natl. Acad. Sci. USA. 2011. 108:17933-38.

 

Immunofluorescence staining for rat NIS expression:

HelaH1 cells stably-expressing (left) rat NIS or (right) human NIS were permeabilized and stained with anti-rat NIS antibody (1:500 dilution) followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody and Hoechst 33342 to stain nuclei. Cell photos were taken at 200X magnification.

Immunoblotting for rat NIS protein:

Total protein (10 mg) from HeLaH1 cells stably-expressing rat (lane 1), dog (lane 2), pig (lane 3), or rhesus (lane 4) NIS were subjected to SDS-PAGE and western blot analysis using anti-rat NIS antibody (1:3,000 dilution) and HRP-conjugated anti-rabbit secondary antibody. The top band (~75-90 kDa) represents the hyperglycosylated form of rNIS, while the bottom band (~60 kDa) represents the hypoglycosylated form of rNIS. Data from Dr. Nancy Carrasco's (Yale University) laboratory.