Description: Affinity purified rabbit polyclonal antibody to human (ETNL) NIS 

Antigen: Synthetic peptide GHDGGRDQQETNL corresponding to residues 631-643 of the human sodium iodide symporter, hNIS (ref.1).

Detects both the native and denatured forms of human NIS. This antibody recognizes an intracellular C-terminal epitope. This antibody does not detect rhesus, mouse, pig or dog NIS.

Host Species: Rabbit

Isotype: Not applicable. Polyclonal affinity purified antibody.

Concentration: 1.5 mg/mL

Species reactivity: Human

Tested Applications (see references below):

• Flow cytometry (1:500 dilution) Refs: 2,3
• Immunofluorescence (1:500 dilution) Refs: 3,4
• Immunohistochemistry (1:5,000 dilution) Refs: 5,6
• Western Blotting (1:1,000 to 1:5,000 dilution) Refs: 1,5

Selected Product References:

1. Wapnir et al. J Clin Endocrinol Metab. 2003. 88-1880-1888.

2. Li et al. FASEB J 2003. 27: 3229-3238.

3. Paroder et al. 2013. J Cell Sci. 126: 3305-3313

4. Dohan et al. Mol Endocrinol 2006. 20: 1121-1137

5. De la Vieja et al. 2004. J Cell Sci. 117: 677-687

6. De la Vieja et al. 2005. Mol Endocrinol 19: 2847-2858

7. Tazebay et al. 2000. Nat Med. 6: 871-878

 

All testing were performed by scientists at Imanis Life Sciences.

Immunofluorescence staining for human NIS expression:

HelaH1 cells stably-expressing (left) human NIS or (right) rhesus macaque NIS were fixed, permeabilized and stained with anti-human ETNL NIS antibody (1:500 dilution) followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody and Hoechst 33342 to stain nuclei. Cell photos were taken at 200X magnification.

Immunoblotting for human NIS protein:

Total protein (20 ug) from parental HeLaH1 (lane 1), HeLaH1 cells stably-expressing human (lane 2), mouse (lane 3), rhesus macaque (lane 4), pig (lane 5) or dog (lane 6) NIS were subjected to SDS-PAGE and western blot analysis using anti-human ETNL NIS antibody (1:3,000 dilution) and HRP-conjugated anti-rabbit secondary antibody. A 90kDa band of NIS was detected. 

Flow cytometry:

(Left panel) parental HelaH1 cells or (right panel) HelaH1 cells stably expressing human NIS linked to enhanced green fluorescent protein (GFP) were fixed, permeabilized, and stained with anti-human ETNL NIS antibody (1:500 dilution) followed by an Alexa Fluor-594 conjugated anti-rabbit secondary antibody. Samples were subjected to flow cytometry analysis.